Metabolic profiling analysis of the vitamin B 12 producer Propionibacterium freudenreichii

Lipase and Esterase Activities of Propionibacterium freudenreichii subsp. freudenreichii.

The lipase and esterase activities of eight strains of dairy Propionibacterium freudenreichii subsp. freudenreichii were studied. A lipase activity was detected on whole cells and in the culture supernatant. The highest activity was expressed at 45 degrees C and pH 6.8. An esterase activity was also detected in the culture medium. The electrophoresis of the intracellular fractions of the cells ...

Utilization of coenzyme A by Propionibacterium freudenreichii.

Aminopeptidase activities of Propionibacterium freudenreichii dairy isolates

This study was undertaken to achieve more information on the aminopeptidase activities expressed in Propionibacterium freudenreichii strains naturally occurring in milk and dairy products. Fifty four strains belonging to both subspecies freudenreichii and shermanii were analyzed for activity towards different amino acyl β-naphthylamide (βNA) derivatives. The ability to efficiently hydrolyze ami...

In situ production of active vitamin B12 in cereal matrices using Propionibacterium freudenreichii

The in situ production of active vitamin B12 was investigated in aqueous cereal-based matrices with three strains of food-grade Propionibacterium freudenreichii. Matrices prepared from malted barley flour (33% w/v; BM), barley flour (6%; BF), and wheat aleurone (15%; AM) were fermented. The effect of cobalt and the lower ligand 5,6-dimethylbenzimidazole (DMBI) or its natural precursors (ribofla...

Some metabolic aspects of vitamin B 12 deficiency in sheep.

I. Studies were made with pair-fed vitamin Blz-deficient and vitamin B12-treated or cobalt-treated ewes fed a Co-deficient diet in cages. Measurements were made of body-weight changes and some observations were made of energy and nitrogen metabolism. The effects of oral Co on energy and nitrogen metabolism were examined in sheep fed the Co-deficient diet, but not yet deficient of vitamin B,, in...